|
Fig. S2
Lysosomal acidification during fin regeneration.
(A) Experimental scheme. Red two-headed-arrows indicate LysoTracker treatment, which was applied 15 min before observation. (B-F’) Images of bright-field and fluorescence microscopy, and quantification of LysoTracker fluorescence intensities at 0, 3, 6, 12, and 24 hpa (n = 5). Black boxed areas in B-F are enlarged in B’-F’, respectively. The LysoTracker fluorescence intensities in red boxed areas were measured (B-F’). Representative images (B’-F’) used for quantification are shown in G. White dashed lines indicate the amputation planes. Scale bars: 1 mm (B-F) and 500µm (B’-F’). n.s.: not significant. ***p < 0.001 by Student’s t test. Error bars represent the standard error.