ZFIN Image: LLeras Forero et al., 2018, Fig. 1-S2

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Figure Caption

Fig. 1-S2

Disruption of the segmentation clock in tbx6, her1;her7, her1;her7;tbx6 and her1;her7;hes6 mutants.

(A-H') In situ hybridization for segmentation clock markers her1 and deltaC in tbx6^−/−, her1^−/−;her7^−/−and her1^−/−;her7^−/−;tbx6^−/−. her1 (B and B’) and deltaC (F and F’) oscillate in the posterior PSM of tbx6^−/−. her1 (C and C’) and deltaC (G and G’) do not oscillate in her1^−/−;her7^−/−. (D and D’, H and H’) her1 and deltaC do not oscillate and their expression is restricted to the posterior PSM in her1^−/−;her7^−/−;tbx6^−/−. (I-P') Comparison of clock markers her1, her7 and deltaC and segmental output marker mespb between her1^−/−;her7^−/− and her1^−/−;her7^−/−;hes6^−/−. her1, her7 and deltaC expression domains are indistinguishable in her1^−/−;her7^−/− (I and I’, J and J’, K and K’) and her1^−/−;her7^−/−;hes6^−/− (M and M’, N and N’, O and O’). mespb expression in the anterior PSM is equivalently disordered in both mutants (L and L’, P and P’). Embryos are 13.5 hpf (10-somite stage). Scale bar in A applies to A-H’. Scale bar in I applies to I-P’. Scale bars are 100 µm.

Acknowledgments

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