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Fig. 5
arxa levels are regulated by activity and the NMDAR. A–A’’, foxP2.A.2:egfp-caax and arxa are coexpressed in early development as the TCPT axons extend toward the midline. Sections, rostral top, of arxa in situ expression and Tg(foxP2.A.2:egfp-caax) α-GFP immunohistochemistry. Red boxed inset shows sense probe control for arxa. B, arxa expression is reduced following blockade (APV) or knock-down (CRISPR) of NMDAR or inhibiting activity (tricaine). qRT-PCR at 48 hpf, experimental triplicates; n > 10 embryos each group. Relative log2 fold change calculated relative to wild-type, elf1a standard, SD for each in parenthesis, p values adjusted using Benjamini–Hochberg false discovery rate; **p < 0.01; error bars, SEM.