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Fig. 5
Atrial laterality is established from distinct antero-posterior fields without contribution from the second heart field.
(a, c) Cardiac disc (dotted circle) at 23 ss. (b–b’) Confocal projections of photoconverted kikGR with Tg(myl7:BFP-CAAX) hearts (yellow lines) show that cells from the PDC populate the left side of the atrium while (d–d’) cells from the ADC populate the right side of the atrium (white dotted lines). (b’’, d’’) Single optical planes from b’ and d’, respectively (cardiomyocytes, green arrows; endocardial cells, white arrows). (e) Tg(meis2b-reporter) expression and antibody staining for Isl1 in the 48 hpf heart [meis2b+/Isl1+ (white arrows), meis2b+/Isl1- (green arrows) and meis2b-/Isl1+ (pink arrows) cardiomyocytes]. (f) Confocal projections of control uninjected and isl1 MO injected 48 hpf Tg(meis2b-reporter);Tg(myl7:mCherry) hearts [white lines delineate Tg(meis2b-reporter) expression]. Scale bars: 100 µm.