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Fig. 5
Repairing ability during embryo development at 1k-cell, 8 hpf, 24 hpf and 72 hpf in progenies from untreated sperm and sperm treated with 30 s and 40 s of UV irradiation. (A) 53BP1 and γ-H2AX immunolocalization. Representative confocal images (40×) of whole embryos. Cell nuclei were stained with DAPI. Scale bars: 10 µm. (B) Percentage of pixel intensity per cellular area for γ-H2AX. (C) Percentage of pixel intensity per cellular area for 53BP1. Data are mean±s.e.m. (n=3) (*P<0.05).
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