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Fig. 4
Phenotypes of larvae from untreated sperm and sperm treated with 30 s and 40 s of UV irradiation. (A) Representation of mandibular cartilage from zebrafish embryos at 6 dpf. mc, Meckel's cartilage; pq, palatoquadrate; bh, basihyal; ch, ceratohyal; hs, hyosymplectic; cb, ceratobranchial. Scale bars: 100 µm. (B) Types of malformations observed at 72 hpf. AT, axial torsion; DT, distal torsion; DYS, defective yolk sac; NF, no fin; CE, cardiac edema; NH, no hatching; FP, failed pigmentation; NPF, no pectoral fin. Scale bars: 500 µm. (C) Percentage of total malformations. (D) Percentage of specific malformations. Data are mean±s.e.m. (n=3) (*P<0.05).