ZFIN Image: Bouffard et al., 2018, Fig. 8

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Figure Caption

Fig. 8

In fbl mutants, the S-phase of the cell cycle is affected. (A) PCNA staining in 2dpf wild type (A1) and mutant (A2). In wild-type larvae, proliferative cells were restricted to the periphery of each tectal lobes. In fbl^hi2581Tg PCNA positive cells were observed in almost all tectal cells. (B) EdU incorporation experiments in 2dpf wild type (A1) and mutant (A2) embryos after two hour pulse. In wild-type embryos, EdU-positive cells are restricted to the periphery of the OT while in the fbl^hi2581Tg mutant embryos EdU-positive cells are spread all over the structure. (C) EdU-positive cells quantification in wild-type (purple) and mutant (pink) embryos at 2dpf. Statistical analyzes have been performed on four samples per conditions, p-value (Mann&Whitney test): 1.00. (D) pH3 staining in 2dpf wild types (E1) and mutants (E2) embryos. Similar abnormal patterns in mutants as for EdU incorporation experiments. (E) pH3-positive cells quantification in wild-type (purple) and mutant (pink) embryos at 2dpf. Statistical analyzes have been performed on four samples per conditions, p-value (Mann&Whitney test): 0.53 (E) embryos. Gray: DAPI staining, Pink: EdU, pH3 or PCNA staining. Scale bar: 50 µm. Anterior is to the left.

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Acknowledgments

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Reprinted from Developmental Biology, 437(1), Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., Corre, R., Bourrat, F., Joly, J.S., Jamen, F., Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina, 1-16, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.