ZFIN Image: Kenyon et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

Binary transgenic zebrafish model for regulatory profiling of myeloid cells. (A) Schematic of myeloid nuclear biotagging system. When biotagging effector transgenic zebrafish line ubiquitously expressing Avi-tagged Rangap for biotinylation of nuclei is crossed to biotagging driver line expressing BirA in myeloid-specific manner, only the macrophage or neutrophil nuclei will be biotinylated. (B) Transgene expression in macrophage-specific BirA driver, TgBAC(mpeg1:BirA-Citrine)^ox122, is amplified with anti-GFP–Alexa-Fluor-488 to show expression in macrophages. (C) TgBAC(mpx:BirA-Citrine)^ox121 biotagging transgenic driver shows neutrophil-specific expression. (D,E) Projection of confocal microscope images of Tg(bactin:Avi-Cerulean-Rangap)^ct700a shows ubiquitous expression of the biotag effector specifically on nuclei, across somite region of the embryo, with (D) and without (E) bright field background. The images in D and E are taken in different embryos. (F,G) Tailfins of transgenic fish transected at 3 dpf show responding macrophages in mpeg1:BirA-Citrine (F) and neutrophils in mpx:BirA-Citrine (G) larvae at 5 hpi or 1 hpi, respectively, as indicated by green arrows.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.