ZFIN Image: Dobrzycki et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

Runx1 mutants have significantly reduced levels of dnmt3bb.1 mRNA detected by ISH. (A) Representative images of ISH for dnmt3bb.1 in 33 hpf wild-type (blue), runx1^+/W84X (green) and runx1^W84X/W84X (orange) embryos, showing the expression in the dorsal aorta. The top panel indicates the location of the zoomed regions in a wild-type embryo. (B) 2% agarose gel showing representative genotypes of wild-type (WT), heterozygous (HET) and mutant (MUT) runx1 embryos, distinguished by RFLP. Yellow: wild-type 214 bp+124 bp bands, pink: 338 bp mutant band. First lane from the left: 100 bp DNA ladder. (C) Pixel intensity values of dnmt3bb.1 mRNA in runx1^W84X/W84X embryos (n=36) are significantly decreased compared to wild type (n=32) and heterozygotes (n=62) (ANOVA, P<0.001). The coefficients of variation are 24%, 22% and 21% for wild-type, heterozygote and mutant groups, respectively. Blue, green and orange data point correspond to the example images from panel A. The bars represent mean±s.d. ***P<0.001 (Games-Howell post-hoc test).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open