ZFIN Image: Williams et al., 2018, Fig. S7

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Figure Caption

Fig. S7

Reduction of EpCAM does not suppress cell polarity defects in MZudu-/- gastrulae

a-b) Live images of otic vesicles in WT embryos at 30hpf either (a) uninjected or (b) injected with 1ng epcam MO. Arrowheads mark otoliths (or lack thereof). Approximately 36% of epcam MO-injected embryos from three independent trials lacked otoliths as shown in b, an additional 56% exhibited smaller otoliths (both phenotypes are observed in epcam mutants²). c-f) Quantification of axial mesoderm cell orientation (e) and elongation (f) in epcam overexpressing WT gastrulae. Asterisks indicate significant differences compared to WT controls (Kolmogorov-Smirnov (e) and Mann-Whitney (f) tests, **p<0.01, ***p<0.001, ****p<0.0001). Black bars are median values in e, mean values in f; medians and means of WT are shown as gray bars in e-f. g-n) Quantification of axial mesoderm cell orientation and elongation in control MZudu-/- siblings (g-j) and MZudu-/- injected with 1ng epcam MO (k-n) at the time points indicated. Graphs and color coding as in Fig. 3. Bars are median values in i, m; bars are mean values in j, n. N indicates the number of embryos analyzed, scale bar is 50μm.

Acknowledgments

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