ZFIN Image: Huang et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

per2 mutant zebrafish larvae exhibited normal retinal morphology and abnormal photoreceptor ribbon synapses. (A,B) AB-WT and per2^−/− zebrafish larval retinas revealed relatively normal retinal morphology by IHC. Images are LSCM presentations. Larvae at 5 dpf were stained with zpr1 (green) and propidium iodide (PI; red), which label double cones and nuclei (n = 6 animals per genotype). Scale bar = 40 μm. (C–F) per2 mutant zebrafish larvae exhibited abnormal ribbon synapses. (C) In the AB-WT retina, synaptic ribbons (arrows) are associated with the presynaptic membrane via an arciform density (asterisks). (D) In the per2^−/− retina, synaptic ribbons in most of the pedicles appear to be floating in the cytoplasm and are unassociated with an arciform density and the presynaptic membrane. AB-WT (E) had relatively more normal ribbon synapses (asterisks) than those of the per2 mutant (F; n = 3 retina per genotype). Scale bar = 1 μm. (G,H) Expression of cacnf1a and syjn1 was reduced in mutant compared with WT retinas (5 dpf, n = 10 animals per genotype, *P < 0.05 and ***P < 0.001, unpaired two-tailed t-tests). Data represent the mean ± SEM.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Behav. Neurosci.