ZFIN Image: Trivedi et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Measuring a twofold difference in mRNA levels. (A) Two-channel imaging of citrine (red channel, Alexa 647) and desma (green channel, Alexa 546) target mRNAs in homozygous Gt(desma-citrine)^{ct122a/ct122a} embryos and heterozygous Gt(desma-citrine)^ct122a/+ embryos. Confocal microscopy: 0.7�0.7?�m pixels. Whole-mount zebrafish embryos fixed at 26?hpf. Depicted regions are analyzed in B-D. (B) Normalized signal for citrine (red) and desma (green) targets in homozygous and heterozygous embryos (mean�s.d. via uncertainty propagation, n=3 embryos). (C) Ratio of citrine target in homozygous versus heterozygous embryos (mean�s.d. via uncertainty propagation, n=3 embryos). (D) Normalized signal for 2�2�2?�m voxels within the selected regions of A. See section S2.4 in the supplementary material for additional data.

Acknowledgments

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