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Fig. 5
Immunohistochemistry with WT1 on regenerative heart (a bar = 300 µm; b bar = 150 µm; c bar = 75 µm; d bar = 45 µm; e bar = 50 µm; f bar = 45 µm; g bar = 150 µm). At 2 days, (a) there is a strong reactivity in the epicardium surrounding the organ. This positivity is present also in EPCs surrounding the clot (b) and in cells inside the clot (c). At 3 dpa, (d) the epicardium is reactive, but at 7 dpa (e) the signal in some epicardial portions is lower as compared with the parts next to the RC (f). In g, the control staining (bar = 75 µm). Ep epicardium
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