ZFIN Image: Brown et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

The NA/EE-mixture induces morphological damage in zebrafish embryos at 6 hpf, 24 hpf, 48 hpf and 72 hpf in a stage-sensitive manner. Embryos at 6 hpf, 24 hpf, 48 hpf and 72 hpf were treated with either DMSO or NA/EE-mixture (NA 12.5 μg/mL + EE 25 ng/mL), incubated for 24 hours then fixed and imaged. (A–H) Embryos at 6 hpf (A,B), 24 hpf (C,D), 48 hpf (E, F), 72 hpf (G,H) treated with DMSO (A,C,E,G) or NA/EE-mixture (B,D,F,H; n > 15 for all time points and conditions). Note: twisted spine (grey arrowhead); smaller eye (white asterisk); yolk sac anomalies/oedema (grey arrow), bent spine (black asterisk) and pericardial defect (black arrowhead). Scale bars: 250 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.