IMAGE

Fig. 3

ID

ZDB-IMAGE-180620-49

Genes

gh1
rag1

Publication

Cai et al., 2018 - Knockout of zebrafish interleukin 7 receptor (IL7R) by the CRISPR/Cas9 system delays retinal neurodevelopment

All Figures

Figures for Cai et al., 2018

Image

Figure Caption

Fig. 3

The validation of the il7r^−/− mutant by western blotting and whole-mount in situ hybridisation.

a The expression of IL7R protein in WT and il7r^−/− larvae at 4 dpf. Note that no IL7R protein is detected in il7r^−/− larvae. b The images of whole-mount in situ hybridisation with gh1 (arrowheads) and rag1 (arrows) mRNA probes in WT and il7r^−/− larvae at 4 dpf. c-d The statistical analysis of gh1-positive area (c) or rag1-positive area (d) between WT and il7r^−/− larvae. Note that rag1 expression is significantly decreased in il7r^−/− larvae. Results are represented as means ± SEM, ***P < 0.001. The upper four panels in b: dorsal view. The lower two panels in b: dorsal is up, and rostral is left. Scale bar in b: 100 μm

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.