ZFIN Image: Hartill et al., 2017, Fig. 6

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Figure Caption

Fig. 6

DNAAF1-dependent left-sided asymmetric expression of RUVBL1 at the zebrafish Kupffer’s vesicle: (A) in situ hybridization (ISH) of Ruvbl1 expression at Kupffer’s vesicle (thin dashed black line) in wild-type heterozygous dnaaf1^+/− and mutant homozygous dnaaf1^−/− zebrafish embryos, at the 5 to 9 somite stages, visualized from dorsal views as indicated in the top left inset. Localized expression is indicated by arrowheads, and the mid-line of the notochord is shown by the thick dashed black line. Left (L) and right (R) sides are indicated. (B) Bar graph shows a significantly higher proportion of wild-type zebrafish (+/−) have left-sided localisation of ruvbl1 when compared to diffused central localization in mutant (−/−) embryos (P = 0.0023, unpaired Student’s t-test). (C) Additional examples of ruvbl1 expression at Kupffer’s vesicle in unaffected dnaaf1^+/⁻ heterozygous and dnaaf1^−/− affected mutant zebrafish embryos.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.