|ZFIN ID: ZDB-IMAGE-180522-30|
Generation of second nr2f1a deletion allele. (A) Schematic of deletion in nr2f1a exon 2 induced from the CRISPR/Cas9. (B) Sequence of the 49-bp deletion in the nr2f1aci1009 mutant allele. (C) Schematic indicating the predicted truncation caused by the out of frame deletion. (D,E) WT and nr2f1a ci1009-/- mutant embryos at 48 hpf. Arrow indicates blood pooling and edema on yolk.
|Acknowledgments:||ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.|