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Fig. 3
The loss of Fer kinase function resulted in the loss of early anterior erythrocyte formation and the loss of circulation. o-Dianisidine staining showed the slightly higher levels of erythrocyte heme groups in the posterior trunk region of fer-MO1 embryos at 30 somites (~28 hpf) (C, control-D), but not in the anterior (A), as is observed in control embryos (B). The staining was no longer present in the posterior region at 48 and 72 hpf in fer-MO1 ((E,G) respectively) but regained anterior staining, indicating that later erythrocyte production is functional but no circulation is taking place (control F and H, 48 and 72 hpf respectively).