ZFIN Image: Lagendijk et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Inhibiting Vegf signaling impairs linearisation and VE-cadherin tension changes during arterial maturation. (a, b) Junctional morphology of ECs in the DA (Venus, grey) in uic (top) and kdr/kdrl morphants (bottom) at 2?dpf (a) and 3?dpf (b). Scale bar?=?10??m. c Number of ECs quantified at 2?dpf (uic n?=?9, kdr/kdrl MO n?=?6 embryos) and 3?dpf (uic n?=?10, kdr/kdrl MO n?=?7 embryos). Error bars represent mean?�?s.d.; 2?dpf uic?2?dpf kdr/kdrl MO ****p?<?0.0001 from unpaired two-sided t test, 3?dpf uic?3?dpf kdr/kdrl MO **p?=?0.0068 from unpaired two-sided Mann?Whitney test. d Junctional linearity index of junctions in uic and kdr/kdrl morphants at 2?dpf and 3?dpf. Error bars represent mean?�?s.d.; 2dpf uic?3dpf uic ****p?<?0.0001; 2?dpf kdr/kdrl MO?3dpf kdr/kdrl MO, p?=?0.0862 from unpaired two-sided Mann?Whitney test. e, f Ratio-metric FRET values of uic and kdr/kdrl morphants at 2?dpf e (uic n?=?74 junctional ROIs from n?=?9 embryos, kdr/kdrl MO n?=?86 junctional ROIs from n?=?6 embryos) and 3?dpf f (uic n?=?62 junctional ROIs from n?=?10 embryos, kdr/kdrl MO n?=?58 junctional ROIs from n?=?7 embryos). Error bars represent mean?�?s.d.; ***p?=?0.0007; *p?=?0.0391 from unpaired two-sided t test. g Ratio-metric FRET values from 1% DMSO controls (n?=?48 junctional ROIs from n?=?6 embryos) versus SL327 (4??M/1% DMSO)-treated embryos (n?=?66 junctional ROIs from n?=?10 embryos). Treatment from 20 hpf to 3?dpf. Error bars represent mean?�?s.d.; ****p?<?0.0001 from unpaired two-sided t test. h Junctional linearity index in 1% DMSO treated controls (n?=?32 junctional ROIs from n?=?6 embryos) versus SL327 (4??M/1% DMSO)-treated embryos (n?=?56 junctional ROIs from n?=?10 embryos). Error bars represent mean?�?s.d.; **p?=?0.0045 from unpaired two-sided Mann?Whitney test. i Junctional morphology of ECs in the DA (Venus, grey) at 3?dpf in 1% DMSO and SL327 (4??M/1% DMSO) treated embryos at 3?dpf. Scale bar?=?10??m. j Ratio-metric FRET values from uic (n?=?45 junctional ROIs from n?=?8 embryos) versus kdr/kdrl morphants incubated in 1% DMSO as a control (n?=?43 junctional ROIs from n?=?9 embryos) and kdr/kdrl Y-27632-treated morphants (50??M/1% DMSO, n?=?40 junctional ROIs from n?=?9 embryos) at 2?dpf. Error bars represent mean?�?s.d.; uic?kdr/kdrlMO?+?DMSO ****p?<?0.0001; kdr/kdrlMO?+?DMSO ? kdr/kdrlMO?+?Y-27632 ***p?=?0.0005 from unpaired two-sided t test

Acknowledgments

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