PUBLICATION
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
- Authors
- Lagendijk, A.K., Gomez, G.A., Baek, S., Hesselson, D., Hughes, W.E., Paterson, S., Conway, D.E., Belting, H.G., Affolter, M., Smith, K.A., Schwartz, M.A., Yap, A.S., Hogan, B.M.
- ID
- ZDB-PUB-171111-6
- Date
- 2017
- Source
- Nature communications 8: 1402 (Journal)
- Registered Authors
- Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Hesselson, Daniel, Hogan, Ben M., Paterson, Scott, Smith, Kelly
- Keywords
- none
- MeSH Terms
-
- Actomyosin/physiology
- Animals
- Antigens, CD/genetics
- Antigens, CD/physiology*
- Aorta/embryology
- Biomechanical Phenomena
- Cadherins/genetics
- Cadherins/physiology*
- Fluorescence Resonance Energy Transfer
- Intercellular Junctions/physiology
- Mechanotransduction, Cellular/physiology
- Molecular Imaging
- Mutation
- Neovascularization, Physiologic/genetics
- Tensile Strength/physiology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 29123087 Full text @ Nat. Commun.
Citation
Lagendijk, A.K., Gomez, G.A., Baek, S., Hesselson, D., Hughes, W.E., Paterson, S., Conway, D.E., Belting, H.G., Affolter, M., Smith, K.A., Schwartz, M.A., Yap, A.S., Hogan, B.M. (2017) Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nature communications. 8:1402.
Abstract
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping