Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
- Lagendijk, A.K., Gomez, G.A., Baek, S., Hesselson, D., Hughes, W.E., Paterson, S., Conway, D.E., Belting, H.G., Affolter, M., Smith, K.A., Schwartz, M.A., Yap, A.S., Hogan, B.M.
- Nature communications 8: 1402 (Journal)
- Registered Authors
- Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Hesselson, Daniel, Hogan, Ben M., Paterson, Scott, Smith, Kelly
- MeSH Terms
- Antigens, CD/genetics
- Antigens, CD/physiology*
- Biomechanical Phenomena
- Fluorescence Resonance Energy Transfer
- Intercellular Junctions/physiology
- Mechanotransduction, Cellular/physiology
- Molecular Imaging
- Neovascularization, Physiologic/genetics
- Tensile Strength/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- 29123087 Full text @ Nat. Commun.
Lagendijk, A.K., Gomez, G.A., Baek, S., Hesselson, D., Hughes, W.E., Paterson, S., Conway, D.E., Belting, H.G., Affolter, M., Smith, K.A., Schwartz, M.A., Yap, A.S., Hogan, B.M. (2017) Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nature communications. 8:1402.
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes