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Fig. 4 The paralogous zebrafish ?Ba- and ?Bb-crystallin promoters produced similar, but distinct, GFP expression profiles. Zebrafish ?Bb-crystallin has the same syntenic relationship with Hspb2 as mouse ?B-crystallin, although the intergenic region between the two genes is much larger in the zebrafish (A). The zebrafish ?Ba-crystallin paralog has moved to a separate chromosome. Both zebrafish paralogs produced GFP expression most often in notochord (B) and skeletal muscle (C). The ?Ba paralog drove expression in these tissues equally while ?Bb was more active in skeletal muscle (D). Expression in lens (E) and extralenticular regions of the eye was more rare. Images shown are representative with the details of GFP expression not differing noticeably between paralogs or the promoter length used.