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Fig. 6
Lurap1 modulates Dvl membrane localisation. Analysis by confocal microscopy of myc-tagged Xdsh subcellular localisation in indicated conditions. a Xdsh is localised predominantly in the cytoplasm, and weakly at the plasma membrane in a WT embryo. b Enhanced Xdsh membrane localisation in the blastoderm cells of a MZlurap1 embryo. c Xfz7 further enhances Xdsh membrane localisation in a MZlurap1 embryo. d Xdsh membrane recruitment by Xfz7 in WT embryo. e Lurap1 prevents Xdsh membrane recruitment by Xfz7. f Lurap1ΔC has no effect on Xdsh membrane recruitment by Xfz7. g, h Graphs show the extent of Xdsh membrane localisation in different conditions. The cells with stronger or equal membrane than cytoplasmic Xdsh staining are counted. Numbers on the top of each bar indicate total cells examined from 6 to 8 embryos in three independent experiments. Scale bar: a–f 20 µm