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Fig. s7 Endocytosis/recycling is blocked in Itga8 MOs and Pcdh15a MOs and mutants. Confocal images (A-G) and quantitative analysis (H-I) of 3dpf (A-D) and 5dpf (E-G) animals incubated for 30-90 min with FM1-43 (red). Phalloidin (green) was used to counterstain the hair cell bundle. H-I: Quantification of total fluorescent intensity per neuromast expressed as a percentage from control (mean±SEM). At least five independent experiments were performed. One-way ANOVA followed by Dunnett’s multiple comparisons test or two-tailed Student’s t test ***p<0.001, **p<0.01, *p<0.05. ns=not significant. J-O: Analysis of FM1-43 uptake after 30 min incubation in superficial J. Cell Sci. 130: doi:10.1242/jcs.206201: Supplementary information Journal of Cell Science • Supplementary information cells from controls (J, M), MOs (K-L) and pcdh15a mutants (N-O). n=neuromast. Scale: A-D: 6µm; E-G: 11µm; J-O: 35µm.