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Kim et al., 2017 - Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

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Fig. s7 OTG inhibits reporter expression in TOPdGFP transgenic zebrafish

Views from animal pole, dorsal is to the right. Patterns of GFP reporter expression in control (A, 10/10, 100%), human OTG mRNA-injected (B, 12/13, 92%), zebrafish otg mRNA- injected (C, 12/14, 86%), or otg MO-injected (D, 18/20, 90%) embryo at shield stage (6 hpf). E-H. OTG overexpression suppresses whereas otg knockdown enhances Wnt8-induced reporter expression. TOPdGFP transgenic embryos were injected with indicated combination of wnt8 (7/7, 100%), human OTG (8/10, 80%), zebrafish otg mRNAs (6/10, 60%) and zebrafish otg morpholino (MO, 14/16, 87%). GFP reporter expression is examined by in situ hybridization.

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