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Fig. 6 AIBP (apolipoprotein A-I [apoA-I]–binding protein) effect on neovessel formation in subcutaneously implanted Matrigel plugs. A, Images of Matrigel plugs retrieved from Apoa1bp−/− or control mice. Matrigel plugs were paraformaldehyde (PFA)-fixed, sectioned, and subjected to hematoxylin and eosin (H&E) staining (B) and CD31 (cluster of differentiation 31 protein) immunohistochemistry (C). Note that substantial amount of red blood cells were present in the Matrigel plugs from Apoa1bp−/− mice. D and E, Quantitative real-time polymerase chain reaction (RT-PCR) analysis of endothelial cell (EC) gene markers CD31 and VE (vascular endothelial)-cadherin (VE-Cad). Western blot of Notch intracellular domain (NICD) expression (F), quantification of NICD protein levels in F (G), and qRT-PCR analysis of the Notch downstream gene expression (H) in the Matrigel plugs from Apoa1bp−/− or control mice. Mean±SD; n=4, *P<0.05; **P<0.01. Scale bar: B, 50 μm; C, 100 μm.