Fig. S5

Fig. S5 kdm6ba/bb are not required for the transient depletion of bulk H3K27me3 levels in trabeculating cardiomyocytes. (A-D”) Confocal microscopy immunofluorescence images of sections through the hearts of 3 dpf Tg(kdrl:EGFP) control and kdm6ba/bb-deficient embryos stained with antimyosin heavy chain (red, MF20, myocardium), anti-EGFP (white, endocardium), and anti-H3K27me3 (green) antibodies with Hoechst-stained nuclei in blue (A, B, C, D). Yellow boxed areas in A and C are shown zoomed in B-B” and D-D” respectively with grey-scale single channel images of H3K27me3 staining (B’, D’) and nuclei (B”, D”). Arrowheads indicate myocardial cells with robust (yellow) or depleted (red) bulk H3K27me3 levels. 20 μM and 5 μM scale bars are shown