ZFIN Image: Choi et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Generation of sam2 KO fish using ZFNs. (A) DNA sequencing analysis of two sam2 KO alleles (sam2^cnu1 and sam2^cnu2) with 5-bp and 17-bp deletion, respectively. Yellow mark, Left and Right ZFN-binding regions; red, spacer. (B and B′) Allele-specific genotyping of sam2^cnu1 and sam2^cnu2 KO fish by genomic PCR and digestion for BtgI site (CCGTGG) newly created in sam2^cnu1. (C and C′) Normal morphology of sam2^cnu1 KO embryo at 3 d. (D–E′) Projections of Hb efferent axons targeting the IPN in the Et(-1.0otpa:mmGFP)hd1 transgenic sam2^cnu1 KO fish were not visibly affected. Lateral views (D and E) and dorsal views (D′ and E′). dIPN, dorsal IPN; FR, fasciculus retroflexus; lFR, Left FR; lHb, Left Hb; MR, median raphe; rFR, Right FR; rHb, Right HbvIPN, ventral IPN. (Scale bar, 100 µm.)

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Expression / Labeling details

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA