|
Fig. 4
Response of the HBO to asymmetric and symmetric visual stimuli. a Schematic of the two-photon light-sheet imaging setup with stereo-visual stimulation. b Comparison of the gaze-tuned and visual response projection maps. Top: the red and blue colours encode leftward and rightward gaze-tuning Z-score, respectively (N?=?7 fish). Bottom: the red and blue colours encode the visual response Z-score (Supplementary Methods) to unilateral stimulation on the left and right eye, respectively (N?=?11 fish). The HBO circuit (white rectangle) is engaged in both sensory and motor processing. c Coronal sections of the visual response map along the two dotted lines shown in (a). In (b) the dotted rectangle delineates the recorded volume. d Alternated unilateral visual stimulation. e Example traces of left and right HBO. f Trial-averaged response of the HBO over 20 stimulation periods. Shaded regions correspond to left (dark grey) and right (pale grey) illumination. g Bilaterally symmetric 100?ms-long flashes. h Example traces of the right and left HBO. The grey lines indicate the flashes. i Trial-averaged flash-induced responses of each subpopulation (100 flashes). j Left vs. right HBO responses (1311 flashes, N?=?12 fish, r?=??0.5). k Phase-dependent response of each subpopulation to symmetric flashes. l Time-evolution of the HBO oscillatory phase ?(t). The slope of the green line corresponds to the stimulation frequency (period T stim?=?10?s). The HBO is entrained at half the frequency of the stimulation (period 2T stim). m PDF of the HBO stimulation phase offset ??. The inset shows the PDF of the HBO phase at times where the flashes were delivered. Notice that in (f), (i) and (k), the error bars indicate the s.e.m