Fig. 3
Pericardial edema and circulation defect in GB9 treated embryo. (A?C) At 48 hpf, representative edema fish (arrow in (B)) and quantitative results showed 60% of GB9 treated embryo (n = 20) with mild to severe pericardial edema compared to untreated control (n = 19). (D,E) GB9 treatment in transgenic Tg(gata1:dsRed) embryos with dsRed-labeled blood cells showed slow to no circulation at 52 hpf (E) compared to wild-type fish (D). (F) Circulation defects at the ISV/DLAV or slow to lose axial circulation of the aorta/vein in the trunk region are quantified in control (n = 18) and GB9 treated embryo (n = 18) at 52 hpf. The scale bar in (A,B) is 500 ?m, and in (D,E) represents 200 ?m.