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Fig. 4
Representative long-axis (A), short axis (B) brightness mode (B-mode) view of adult zebrafish heart.
Zebrafish were anesthetized, imaged and the long axis mode was considered to calculate the ventricular length (A), while short axis was considered to calculate ventricular area (B) (represented by blue line). Blood flow from atrium to ventricle through the atrioventricular valve are indicated by color (blue) in panel C using color flow Doppler mode and heart rate measurements were calculated by number of heart beats per 10 s during the B-mode ultrasound video loop, and converted to beats per minutes (bpm).
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