ZFIN Image: Quintana et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Inhibition of isoprenoids and cholesterol has not effect on sonic hedgehog (Shh) activity.

(A-C) Whole mount in situ hybridization (ISH) was performed at 3 days post fertilization (protruding mouth stage) (dpf) with embryos treated with 0.01% DMSO (vehicle), 8uM lonafarnib (Lona) or 2.5 uM Ro 48 8071 (Ro-48). (DMSO n = 10, Ro-48 n = 13, and Lona n = 16). (D) Sybr green based quantitative real time PCR was performed on hmgcs1 mutant embryos (Homozygous MT) or their wildtype siblings (WT Sibling) at the indicated days post fertilization (DPF) to detect mRNA expression of gli1. Error bars represent standard deviation of technical replicates.

Figure Data

Expression / Labeling details

Acknowledgments

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