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Fig. 4
miR-145 is required for hepatic outgrowth.
A WISH assay was used for determination of the liver developmental markers hhex at 30 hpf (A-C, dorsal view), sePb at 50 hpf (D-F, dorsal views), prox1 at 72 hpf (G-I, dorsal views) and fabp10 at 96 hpf (J-L, dorsal views) in control, miR-145 inhibitor-injected and miR-145 mimic-injected embryos. The arrowhead indicates liver. The expression levels of hhex, sePb, prox1 and fabp10 were examined using qPCR (M). The ef1a expression level was served as an internal control (*P < 0.05, t-test). Twenty whole embryos per experimental group were used and three independent replicates were performed.