ZFIN Image: Yan et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Cell proliferation analysis of kras^V12- and Myc-induced carcinogenesis.

7?dpf wild type (WT), kras+ or Myc+ larvae were treated with 10??M SU5402, 10??M IWR1 or 10??M cyclopamine in the presence of 10??g/ml Dox. Cell proliferation was analyzed by immunohistochemical staining with PCNA primary antibody. (A?D) Representative liver image of 7?dpf WT larvae. (E?H) Representative liver image of 7?dpf kras+ larvae. (I?L) Representative liver image of 7?dpf Myc+ larvae. (M) Statistical analysis of numbers of proliferating cells in the livers of kras+ larvae. (N) Statistical analysis of numbers of proliferating cells in the livers of Myc+ larvae. N?=?20 from each groups; statistical significance: *p?<?0.05, Scale bar?=?20??m.

Acknowledgments

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