ZFIN Image: Lu et al., 2017, Fig. 3

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Figure Caption

Fig. 3

The thickness of ONL decreased and the apoptosis of retinal cells increased in the EYS^?/? zebrafish.

(A) Retinal histology analysis of WT and EYS^?/? zebrafish. Retinal sections of EYS^?/? zebrafish stained with hematoxylin/eosin (HE) at indicated ages. RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: 50??m. (B) TUNEL staining between WT and EYS^?/? zebrafish. Scale bars: 50??m. (C) Comparison of the ONL thickness between WT and EYS^?/? zebrafish at indicated ages (n?=?3). Quantitative analysis revealed a significant reduction of the ONL thickness at 3?mpf (P?=?0.0479), 4?mpf (P?=?0.0258), 8?mpf (P?=?0.0122), 16?mpf (P?=?0.0272) in the EYS^?/? zebrafish. (D) Quantification of TUNEL positive cells in WT and EYS^?/? zebrafish at indicated ages (n?=?3). Quantitative analysis revealed a significant increase of TUNEL positive cells at 8?mpf (P?=?0.0341), 16?mpf (P?=?0.0028) in the EYS^?/? zebrafish.

Figure Data

Phenotype details

Acknowledgments

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