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Fig. S2
The activation of maternal Wnt/ß-catenin signalling is either unaffected or reduced after VAb@2cell. (A-I) Analysis by ISH of gsc, dha, and chd expression at sphere-dome (A-F) and 50% epiboly (G-I) stages. Animal pole views with dorsal region on the top. (J-L) Immunofluorescence staining of ß-catenin nuclear localisation (arrowheads) in intact and VAb@2cell embryos at 3.5 hpf. Scale bars: (A-I) 250 μm; (J-L) 20 μm.
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