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Fig. S11
Somite number is reduced in vrtn morphants or mutants. (A-D) Specificity of the vrtnMO. Injection of vrtnMO efficiently blocked the translation of the artificial mRNA where the vrtnMO-targeting 5' leader sequence is fused with vrtn-GFP. It has no effect on the artificial mRNA where only vrtn coding region is fused with GFP sequence. (E,F) Phenotypes of uninjected and vrtnMO-injected embryos at 30 hpf. (G) Analysis of the number of somite boundary at 36 hpf after ISH using dystrophin as a marker. (H,I) Statistical analysis of somite number in vrtn morphants and mutants. Horizontal lines represent the mean values ± s.d. from four batches of embryos. Scale bars: (A-D) 400 μm; (E-G) 250 μm.