ZFIN Image: Zhao et al., 2015, Fig. 4

Image

Figure Caption

Fig. 4

Dorsal, lateral and anterior projections of a 33 hpf Tg(enpep:rtTA; P_Tight:CD2V;P_Tight:GEpacmC) zebrafish embryo expressing both CD2V sensor and GEpacmC sensor, before and after being treated with 3?mM EGTA, 100??M BAPTA-AM and 10??M ionomycin for 2?hours to decrease cellular Ca²⁺ level.

(a,e) False color fluorescence intensity projections before and after treatment, with 405-blue channel in blue, 488-green channel in green and 561-red channel in red. (b,f) 405-blue channel fluorescence lifetime (CFP lifetime) increased by 0.10?ns after treatment. (c,g) 488-green channel fluorescence lifetime (intensity weighted average of Venus and GFP lifetimes) was not affected by treatment. (d,h) Recovered GFP lifetime in GEpacmC sensor by the triple-channel intensity lifetime analysis. GFP lifetime was not changed by the treatment. (i) Average CFP lifetimes in 6 segments of kidney tubules divided equally, before and after the Ca²⁺ treatment. Despite significant variations in average CFP lifetimes between segments, CFP lifetimes in all segments increased after the treatment. (j) Average recovered GFP lifetimes in 6 tubule segments, before and after the Ca²⁺ treatment. GFP lifetime showed pronounced spatial variation. This spatial pattern of GFP lifetimes was not significantly altered by the Ca²⁺ treatment. Scale bar, 500??m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.