Fig. S7
Bmp activity at 30 hpf in the transgenic line Tg(MBmp:EGFP) and rescue by CoA injection
Representative images of Tg(Bmp:EGFP) embryos not-injected (A, B) and injected with 1.2 pmol/embryo of ST-MO (C, D) or coasy-MO (E, F) at 30 hpf. A net reduction of the fluorescence intensity is evident in coasy-morphants. Results are from one representative experiment with at least 38 embryos out of two independent replicates. CoA (0.5 pmol/embryo) was injected in the craniofacial area of Tg(Bmp:EGFP) morphants at 24 hpf (I) and Bmp activity compared to that of untreated embryos (G, H) at 60 hpf. One representative experiment out of two replicates.