Fig. 1
Injury of the zebrafish tailfin results in recruitment of neutrophils and macrophages to the injury site.
(a) The tailfin of 3?dpf Tg(mpx:EGFP)i114 and Tg(MPEG1:mCherry) embryos were transected (line of transection shown in red) and a region (0.5?mm length from the tip of the body of the fish) was selected in which to count recruited cells. (b) Temporal recruitment of neutrophils (Tg[mpx:EGFP]i114 [i, ii]) and macrophages (Tg[MPEG1:mCherry] [iii, iv]) post-injury was determined. (c) The numbers of inflammatory cells in each tailfin region were quantified. All time points after 0?h were significantly (p???0.05) different to the cell numbers at 0?h. All images at 80x magnification. ?40 fish per group, from 3 independent experiments. Data expressed as ąS.E.M.