Fig. 1
Stmn4 prohibited elavl3 expression in the dorsal midbrain during early embryogenesis.
(a–f) Representative photographs of whole-mount in situ hybridization against stmn4 in zebrafish embryos at designated stages are displayed. stmn4 were highly expressed in the midbrain regions (boxed). A magnified photograph of the boxed regions is shown beneath each panel. (g–j) Representative photographs of double whole-mount in situ hybridization against stmn4 (purple) and elavl3 (red) at designated stages are presented. Arrowheads point to the dorsal midbrain. Scale bars are shown in the panels (a,g) on the right for each row unless specified in (f). Embryonic stages are shown in hours post fertilization (hpf) at the lower left corner in each panel. e: epiphysis; t: telencephalon; TgSN: trigeminal ganglion sensory neurons; vt: ventral tegmentum.