ZFIN Image: Sedykh et al., 2016, Fig. S5

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Fig. S5

Morpholino knockdown of Rsph9 causes structural axonemal defects.

A-H: TEM images of ciliary cross-sections in rsph9 morphant embryos at 1 dpf. Ventral neural cilia with 9+0 axonemes found in the ventral midbrain (A) and spinal cord (B) of uninjected control embryos. Note outer dynein arms (arrow), indicative of ciliary motility. Rsph9-depleted morphants contain aberrant 8+1 cilia in the ventral midbrain (C, D). Pronephric cilia in uninjected controls have 9+2 axonemes (E). Rsph9 morphants contain a mixture of cilia with 9+2 (F), supernumerary central microtubules, e.g. 9+5 (G) and 9+0 (H). I: Summary of TEM analysis of ciliary axonemes in wildtype, control morpholino-injected, and Rsph9 morpholino-injected embryos.

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Acknowledgments

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