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Fig. S1
Single antibody controls for rsph9/ ace-tubulin immunostaining (controls for bleed-through)
Wild type embryos were stained with an acetylated α-tubulin (A) or anti-Rsph9 antibody (B). Embryos were then incubated with DAPI (cyan) to label nuclei. A-A'': pronephric duct in embryo stained for α-tubulin only. Note absence of ciliary staining on the 568 channel when overexposed. B-'': Pronephric duct in embryo stained for Rsph9 only. Note absence of ciliary staining on the 488 channel, when overexposed.
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