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Fig. 6
The defective midline convergence of the PG and the IR in the sox32 morphant at 34 hpf is suppressed by both etv2MO and sclMO.
sox32MO, etv2MO, sclMO, sox32/etv2 double-MOs, sox32/scl double-MOs and STD-MO were injected into either Tg(wt1b:GFP) (A–F,A'–F') or Tg(kdrl:EGFP)s843 (G–L,G'–L') embryos. The injected embryos were subjected to 3β-Hsd staining and analysed for the effects of MOs on the morphology of the kidney delineated by wt1b:GFP (A'–F'), the axial vasculature by kdrl:EGFP (G'–L'), and the IR by 3β-Hsd (A–L). Distances between bilateral IRs, bilateral glomeruli and the outermost edges of paired PCTs in injected Tg(wt1b:GFP) embryos are quantified in (M–O), respectively, with the number of samples indicated in parentheses. The IR, the glomeruli and the PCT are denoted by red arrow, yellow arrows and orange arrowheads, respectively. Histograms with different letters above them are significantly different (ANOVA and Duncan's multiple test, P < 0.05). Scale bar, 50 μm.