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Fig. 3
The phenotypes of the PG and the IR in the pkd2 morphant at 2 dpf.
(A–D,A'–D',A''–D'') The Tg(fli1:EGFP)y1 embryos injected with the pkd2 MO displayed four classes of phenotypes at 2 dpf, which could be categorized according to various types of interrenal morphology. Class I: single IR cluster to the right of the midline (A–A''); Class II: single IR cluster to the left of the midline (B–B''); Class III: bilateral IR clusters immediately adjacent to the midline (C–C''); Class IV: single IR cluster at the midline (D–D''). There appeared to be no defects in glomerular angiogenesis (yellow arrowheads) in all classes. Red arrows indicate IRs. (E,F) Expression of wt1a was detected in the pkd2 morphant as well as the control embryo at 2 dpf. The images of the control embryo and the pkd2 morphant are representative of 23 and 13 samples, respectively. Scale bar, 50 μm.