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Fig. 2
Supervillin is expressed in the mouse and zebrafish ear.
(A) RT-PCR detection of svila and svilc mRNAs in zebrafish hair cells using two primer pairs to detect svila mRNA [RT plus (lanes 1,3), RT minus (lanes 2,4)] and two primer pairs to detect svilc mRNA [RT plus (lane 5,6), RT minus (lane 7,8)]. Svilb [RT plus (lane 9), RT minus (lane 10)] and svild [RT plus (lane 11), RT minus (lane 12)] mRNAs were detected in zebrafish maculae. (B) RT-PCR of Svil mRNA from mouse hair cells [RT plus (lane 1), RT minus (lane 2)]. (C-H) RNA in situ hybridization. Whole mount 4-dpf zebrafish treated with probes antisense to svila (C) and svilc (F) mRNAs. Both genes are expressed in the otocyst (arrowheads). Controls are displayed. Magnified otocysts show svila (D) and svilc (G) are expressed in the anterior macula (AM). Sense-probed controls (E,H). White dashed lines denote the otic vesicles. Yellow boxes show positions of AM hair cells. PC indicates the region of the posterior cristae (out of focus).