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Fig. 5
The HIF2α transcription factor helps regulate ccndx gene expression and subsequent motor neuron development.
(A) MO knockdown of hif2a reduced the olig2 and isl1 signals in motor neurons (panels a, b, e, and f); co-injection of ccndx mRNA partially rescued this phenotype (panels c and g). Panels d and h: graphs showing the qPCR analysis confirmed the results of morphants with signals lower than control MO-injected embryos at 24 hpf. *P < 0.05. Scale bar, 100 mm. (B) A significantly higher proportion of hif2a morphant embryos had lower numbers of GFP-labeled primary motoneurons as compared to control MO-injected embryos (panels a and b) in the Tg:(isl1:GFP) transgenic line. The effect of rescue experiments with ccndx mRNA is shown in panel c. Quantitative data at 24 hpf are shown in panel d. (C) A significantly higher proportion of hif2a morphant embryos exhibited shorter CaP axons branches as compared to control MO-injected embryos (panels a and b). The effect of rescue experiments with ccndx mRNA is shown in panel c. Quantitative data at 24 hpf are shown in panel d. *P < 0.05.