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Fig. 2
Morpholino (MO) knockdown of zccndx affects motor neuron development.
(A) MO knockdown of zccndx reduced the olig2 and isl1 signals in motor neurons (panels a, b, e, and f); rescue experiments performed by co-injecting ccndx mRNA are shown in panels c and g. Panels d and h: graphs showing the qPCR analysis confirmed the results of morphants with signals lower than control MO-injected embryos at 24 hpf. **P?<?0.05. Scale bar, 100?mm. (B) The ccndx morphant showed a significantly higher ratio of embryos with lower numbers of GFP-labeled primary motoneurons than the control MO-injected embryos (panels a and b) in the Tg:(isl1:GFP) transgenic line. The ccndx mRNA recue experiment is shown in panel c. Panel d: the Statistical charts represent the quantitative results of GFP-labeled primary motoneurons cells at 24?hpf. (C) Labeling of primary motor axons with Znp1 antibody revealed that CaP axon branches were shorter in ccndx morphants than in control morphants (panels a and b); the rescue experiment is shown in panel c. Panel d: graphs showing the percentages of morphants with primary motor defects as compared to control MO-injected embryos at 24 hpf in panels d and h. *P?<?0.05.