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Fig. 4
Smad6 knockdown results in reduced cell invasion of MDA-MB-231 cells.
(a) Quantitative PCR (qPCR) analysis of Smad6 mRNA expression in MDA-MB-231 cells infected with control vector, non-targeting shRNA and two Smad6 shRNA constructs, respectively. (b) Quantification of invasive cell numbers in vector control, non-targeting control and Smad6 knockdown MDA-MB-231 cells injected zebrafish larvae. (c) Representative images of 6 dpi zebrafish larvae showing the Smad6 knockdown effect in MDA-MB-231 cell invasion. Scale bar: μm. 100 *0.01 < P < 0.05.
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