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Fig. 4 S1
Single embryos show highly variable mean levels of krox20 expression and boundary sharpness.
(A) Embryos were injected with mRNA coding for the membrane-bound GFP-CAAX and morpholinos (MO) against Crabp2a or Cyp26a1 or mRNA coding for Crabp2a-Myc or Cyp26a1-Myc or water (WT). Ef1α was used as the standard and a homogeneous collection of mRNA from 100 wildtype embryos was used as the reference for the △△Ct method. Experiments were run in triplicates and repeated 4 (four) times. (B) A sharpness index was calculated using the ratio between the measured length of the theoretical sharp boundary and the actual measured length of the boundary of krox20 expression, consistent with previous models (Zhang et al., 2012). In this example, sharpness of the r3-r4 boundary is the ratio of a/b and the index for the r4-r5 boundary is the ratio c/d.