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Fig. 4
Figure 4. Effects of SP600125 on the expression of p21 and p53. (A) After treatment of larvae with 10 μM SP600125 for 2 days, protein extracts were prepared and subjected to western blot assay using antibodies against p21 and p53. β-Actin was included as the control. (p21, unpaired t-test, two-tailed, t = 4.172, df = 4, p = 0.014; p53, unpaired t-test, two-tailed, t = 3.273, df = 4, p = 0.0307).Bars are mean ± SD for three experimental replicates. *p < 0.05. (B) Localization of p21 and p53 genes with whole-mount in situ hybridization in SP600125-treated and untreated larvae.
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